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primescript tm rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript tm rt reagent kit
    Primescript Tm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+tm+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc13629789-106-9-14
    Average 99 stars, based on 89259 article reviews
    primescript tm rt reagent kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: SESN2 promotes ferroptosis in bladder cancer by negatively regulating the NRF2/FSP1 signaling axis
    Article Snippet: Total RNA was extracted from cells and tissues using TRIzol TM Reagent (Cat# 15596026, Thermo Fisher Scientific, USA). .. Reverse transcription of total RNA into complementary DNA (cDNA) was performed using the PrimeScript TM RT Reagent Kit (Cat# RR037A, Takara, Japan). .. Following reverse transcription, qRT-PCR was carried out using the SYBR Premix Ex Taq Kit (Cat# DRR420A, Takara).

    Article Title: The conserved U1 snRNP subunit FgLuc7 regulates sexual development and pre-mRNA processing in fusarium graminearum.
    Article Snippet: The U1 small nuclear ribonucleoprotein (U1 snRNP) is an essential spliceosome component that mediates pre-mRNA splicing.. Although the molecular function of Luc7 proteins is broadly conserved in eukaryotes, whether this core splicing factor acquires developmentally specialized functions in filamentous fungi remains unclear.. Here, we investigated the U1 snRNP-associated protein FgLuc7 in Fusarium graminearum.

    Article Title: Stress-Responsive Protein IFRD1 Protects Assembled Ribosomes via a Ribosome-Salvaging Mechanism
    Article Snippet: The quality of the mRNA was verified with a Nanodrop spectrophotometer (Thermo). .. 500 ng of RNA was reverse transcribed with the PrimeScript TM RT Reagent kit (Takara) following the manufacturer protocol. .. Power SYBR Green Master Mix (Thermo) fluorescence was used to quantify the relative amplicon amounts of each gene (see Supplementary Table 3 for the list of the primers).

    Article Title: The V-ATPase V 0 c' subunit is critical for Candida albicans pathogenicity in oral epithelial infection
    Article Snippet: RNA quality and integrity were determined using a NanoDrop spectrophotometer (Thermo Scientific, USA) and a Bioanalyzer 2100 system (Agilent, USA). .. RNA purification and reverse transcription were performed using a PrimeScript TM RT Reagent Kit (Takara, Shiga, Japan). .. Real-time PCR was performed by a Bio-Rad CFX96 real-time PCR detection system with SYBR Green qPCR mix (TOYOBO, Japan).

    Article Title: Generation of chimeric chickens resistant to infection by avian leukemia virus subgroups A, B, D, E, and K through primordial germ cells
    Article Snippet: .. Total RNA from PGCs and DF-1 were extracted using Trizol (Invitrogen, 15596026), and reverse transcription into cDNA were performed using the PrimeScript TM RT reagent Kit with gDNA Eraser (TaKaRa, RR047A). .. PCR amplification of the DDX4, c-Kit, PRDM14 , and GAPDH genes were carried out using 2 × Taq PCR StarMix (GenStar, A012-B).

    Article Title: Overexpression of endogenous galactolipases and an efflux transporter enhances the secretion of extracellular free fatty acids by Synechococcus elongatus PCC 7942
    Article Snippet: .. One microgram of total RNA was reverse-transcribed into cDNA with the PrimeScript TM RT reagent Kit (Takara Bio Inc.). ..

    Article Title: ROS function during the early phase of vascular regeneration in Arabidopsis.
    Article Snippet: qRT-PCR Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen). .. Total RNA (350 or 1000 ng) was reverse transcribed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Perfect Real Time) (TAKARA). .. qRT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using THUNDERBIRD TM Next SYBR TM qPCR Mix (TOYOBO).

    Polymerase Chain Reaction:

    Article Title: The conserved U1 snRNP subunit FgLuc7 regulates sexual development and pre-mRNA processing in fusarium graminearum.
    Article Snippet: The U1 small nuclear ribonucleoprotein (U1 snRNP) is an essential spliceosome component that mediates pre-mRNA splicing.. Although the molecular function of Luc7 proteins is broadly conserved in eukaryotes, whether this core splicing factor acquires developmentally specialized functions in filamentous fungi remains unclear.. Here, we investigated the U1 snRNP-associated protein FgLuc7 in Fusarium graminearum.

    Synthesized:

    Article Title: The conserved U1 snRNP subunit FgLuc7 regulates sexual development and pre-mRNA processing in fusarium graminearum.
    Article Snippet: The U1 small nuclear ribonucleoprotein (U1 snRNP) is an essential spliceosome component that mediates pre-mRNA splicing.. Although the molecular function of Luc7 proteins is broadly conserved in eukaryotes, whether this core splicing factor acquires developmentally specialized functions in filamentous fungi remains unclear.. Here, we investigated the U1 snRNP-associated protein FgLuc7 in Fusarium graminearum.

    Article Title: Sarcandra glabra genome unveils functional conservation and divergence of O -methyltransferases catalyzing isofraxidin biosynthesis in angiosperms
    Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript TM RT reagent Kit with gDNA Eraser (RR047A, Takara, Japan). .. Gene-specific primers, along with the reference gene (Clathrin adaptor complexes), were designed using SnapGene 6.0.2 software (Dotmatics, USA) and synthesized by SunYa Company (Hangzhou, China) (Supplementary Table ).

    Purification:

    Article Title: The V-ATPase V 0 c' subunit is critical for Candida albicans pathogenicity in oral epithelial infection
    Article Snippet: RNA quality and integrity were determined using a NanoDrop spectrophotometer (Thermo Scientific, USA) and a Bioanalyzer 2100 system (Agilent, USA). .. RNA purification and reverse transcription were performed using a PrimeScript TM RT Reagent Kit (Takara, Shiga, Japan). .. Real-time PCR was performed by a Bio-Rad CFX96 real-time PCR detection system with SYBR Green qPCR mix (TOYOBO, Japan).



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